Public University of Navarre



Castellano | Academic year: 2018/2019
Double degree in Agrifood Engineering and Rural Environment and Innovation of Food Processes and Products from Navarre Public University at the Universidad Pública de Navarra
Course code: 503201 Subject title: MICROBIOLOGY AND PLANT BIOTECHNOLOGY
Credits: 6 Type of subject: Mandatory Year: 2 Period: 1º S
Department:
Lecturers:

Partes de este texto:

 

Module/Subject matter

Bases of Crop Production

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Descriptors

General principles of biology of microorganisms, bacterial genetics and metabolism, Major groups of microorganisms, microbial biotechnology, in vitro Plant tissue culture, micropropagation

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General proficiencies

  •  CB1: Students are able to demonstrate they have acquired knowledge and understanding in a field of study based on the basic foundations gained within their general secondary education together with the support of advanced textbooks and aspects of the latest advances in the field.
  • CB3: Students have the ability to collect and interpret relevant data (generally within their field of study) in order to make judgments which include reflection on relevant issues of social, scientific or ethical nature.

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Specific proficiencies

  •  CE12: Ability to recognize the applications of biotechnology in agriculture and livestock engineering

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Learning outcomes

  •  R1 - To understand the functions of microorganisms in relation to plants and environment for their use in crop production and in agro-industrial processes
  • R2 - To know, understand and apply the molecular underpinnings and biotechnological techniques in the transformation of species of agronomic interest.

     

 

  • RESULTADOS APRENDIZAJE ENAEE:

    ENAEE-11: La capacidad de diseñar y realizar experimentos, interpretar los resultados y sacar conclusiones.

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Methodology

Methodology - Activity Attendance Self-study
A-1 Exposition/Participative Classes  30  20
A-2 Practical classes  30  10
A-3 Cooperative learning activities    7
A-4 Group projects    
A-5 Individual practice and study time    50
A-6 Tutorials    
A-7 Exams and evaluation activities  3  
Total  63  87

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Relationship between formative activities and proficiencies/learning outcomes

Proficiency Formative activity
 CE12  A1, A2, A4, A5, A6, A7, A8
 CB1  A1, A2, A4, A5, A6, A7, A8
 CB2  A1, A2, A4, A5, A6, A7, A8

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Languages

English

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Evaluation

Learning outcome Evaluation system Weight (%) Possibility of resit
  R1, R2  Written test of the theoretical acquired concepts 70%
minimum grade for
weigh in
final score
= 5/10 in this section 
 Recoverable, 
written test
 R1, R2  Written work submitted by the students  25%
minimum grade for
weigh in
final score
= 5/10 in this section 
 Recoverable, 
written test
  R1, R2   Record of attendance  5%  NO

 

To pass the course, it is necessary, but not sufficient, to pass the course exam (score equal to or greater than 5.0) and to attend at least to 80% of the practical classes. Should not justify the practical classes attendance requirements, it will be necessary, but not sufficient, to approve an special practical course exam in addition to the general course exam.

 

The students failing the practical exam, will have the mark of that practical exam as final course mark.

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Contents

- Los principales grupos de microorganismos. Procedimientos metodológicos de trabajo con microorganismos y pautas de crecimiento y control. Estructura genética y metabolismo bacteriano.

- Estructura y síntesis de las proteínas y los ácidos nucléicos. Tecnología del ADN. Las técnicas de trasnformación génica de los microorganismos y en las plantas superiores. Los cultivos transgénicos. Cultivo in vitro.

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Agenda

THEORY

  1.  General Microbiology
    1. Structure microorganisms
      1. Bacteria
      2. Mushrooms
      3. Virus
    2. Metabolism (primary and secondary metabolism)
      1. Primary Metabolism
      2. Secondary metabolism
    3. Growth kinetics of growth and culture media
    4. Elements molecular biology
      1. Structure of genetic material and
      2. Regulation of gene expression in prokaryotes and eukaryotes
    5. Manipulation of genetic material
      1. Transformation
      2. Conjugation
    6. Microbial groups of interest
      1. Proteobacteria
      2. Firmicutes
      3. Actinobacteria
      4. Mushrooms
    7. Relationships between different organisms
      1. Rhizobium
      2. Agrobacterium
      3. Ruminal ecosystem
  2. Industrial microbiology
    1. Products of bacterial origin
    2. Products of fungal origin
    3. Fermenters and bioreactors
    4. Waste treatment
      1. Solid wastes
      2. Liquid wastes
    5. Bioremediation
  3. Plant biotechnology
    1. General micropropagation techniques
      1. Facilities
      2. Culture media
      3. Multiplication by nodal segments or axillary branching
      4. Organogenesis
      5. Somatic embryogenesis
      6. Commercial Applications
    2. Production of virus-free plants
    3. Conservation of germplasm in vitro
      1. Medium-term conservation
      2. Stable long-term cryopreservation
    4. Other applications of plant tissue culture
      1. Protoplast culture and somatic hybridization
      2. Haploid Obtention
      3. Production of secondary metabolic compounds
    5. Transgenic Plants
      1. Concepts and methods of obtention
      2. Agronomic applications marketed

 

PRACTICE

  1. Microbiology
    1. Preparation of culture media
    2. Cultivation of microorganisms
    3. Microscopic observation of microorganisms
    4. bacterial transformation
  2. Industrial biotechnology
    1. Management fermentors
  3. Plant biotechnology
    1. Preparation of culture media
    2. Set up of in vitro culture
    3. Micropropagation of potato
    4. Organogenesis in tobacco
    5. Expression of a reporter gene in transgenic plants

Agenda

THEORY

  1.  General Microbiology
    1. Structure microorganisms
      1. Bacteria
      2. Mushrooms
      3. Virus
    2. Metabolism (primary and secondary metabolism)
      1. Primary Metabolism
      2. Secondary metabolism
    3. Growth kinetics of growth and culture media
    4. Elements molecular biology
      1. Structure of genetic material and
      2. Regulation of gene expression in prokaryotes and eukaryotes
    5. Manipulation of genetic material
      1. Transformation
      2. Conjugation
    6. Microbial groups of interest
      1. Proteobacteria
      2. Firmicutes
      3. Actinobacteria
      4. Mushrooms
    7. Relationships between different organisms
      1. Rhizobium
      2. Agrobacterium
      3. Ruminal ecosystem
  2. Industrial microbiology
    1. Products of bacterial origin
    2. Products of fungal origin
    3. Fermenters and bioreactors
    4. Waste treatment
      1. Solid wastes
      2. Liquid wastes
    5. Bioremediation
  3. Plant biotechnology
    1. General micropropagation techniques
      1. Facilities
      2. Culture media
      3. Multiplication by nodal segments or axillary branching
      4. Organogenesis
      5. Somatic embryogenesis
      6. Commercial Applications
    2. Production of virus-free plants
    3. Conservation of germplasm in vitro
      1. Medium-term conservation
      2. Stable long-term cryopreservation
    4. Other applications of plant tissue culture
      1. Protoplast culture and somatic hybridization
      2. Haploid Obtention
      3. Production of secondary metabolic compounds
    5. Transgenic Plants
      1. Concepts and methods of obtention
      2. Agronomic applications marketed

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Bibliography

Access the bibliography that your professor has requested from the Library.


BASIC BIBLIOGRAPHY:

 

Acceda a la bibliografía que su profesor ha solicitado a la Biblioteca.

 

MICROBIOLOGÍA y BIOTECNOLOGÍA INDUSTRIAL

 Madigan MT, Martinko JM Dunlap, PV y Clark, DP. 2009. Brock: Biología de los microorganismos. Ed. Pearson, Madrid.

 Prescott LM, Harley JP y Klein DA. 2002. Microbiología. McGraw-Hill Interamericana de España. Madrid.

BIOTECNOLOGÍA VEGETAL

 Hurtado DV y Merino ME (eds). 1987. Cultivo de tejidos vegetales. Editorial Trillas, México.

 Lindsey K y Jones MGK. 1992. Biotecnología vegetal agrícola. Editorial Acribia, Zaragoza.

 Serrano M y Piñol MT. 1991. Biotecnología Vegetal. Editorial Síntesis, Madrid.

 Vidalie H (ed). 1986. Cultivo in vitro. Editorial Científica, México.

 Trigiano RN y Gray DJ (eds). 2000. Plant tissue culture concepts and laboratory exercises. CRC Press, Boca Ratón.

 

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Location

Lecture Building (Aulario)

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