Universidad Pública de Navarra - Nafarroako Univertsitate Publikoa
Public University of Navarre
| Course code: 501304 | Subject title: MICROBIOLOGY AND PLANT BIOTECHNOLOGY | ||||
| Credits: 6 | Type of subject: Mandatory | Year: 2 | Period: 1º S | ||
| Department: Ciencias de la Salud | |||||
| Lecturers: | |||||
Contents
THEORY
- General Microbiology
- Structure microorganisms
- Bacteria
- Mushrooms
- Virus
- Metabolism (primary and secondary metabolism)
- Primary Metabolism
- Secondary metabolism
- Growth kinetics of growth and culture media
- Elements molecular biology
- Structure of genetic material and
- Regulation of gene expression in prokaryotes and eukaryotes
- Manipulation of genetic material
- Transformation
- Conjugation
- Microbial groups of interest
- Proteobacteria
- Firmicutes
- Actinobacteria
- Mushrooms
- Relationships between different organisms
- Rhizobium
- Agrobacterium
- Ruminal ecosystem
- Industrial microbiology
- Products of bacterial origin
- Products of fungal origin
- Fermenters and bioreactors
- Waste treatment
- Solid wastes
- Liquid wastes
- Bioremediation
- Plant biotechnology
- General micropropagation techniques
- Facilities
- Culture media
- Multiplication by nodal segments or axillary branching
- Organogenesis
- Somatic embryogenesis
- Commercial Applications
- Production of virus-free plants
- Conservation of germplasm in vitro
- Medium-term conservation
- Stable long-term cryopreservation
- Other applications of plant tissue culture
- Protoplast culture and somatic hybridization
- Haploid Obtention
- Production of secondary metabolic compounds
- Transgenic Plants
- Concepts and methods of obtention
- Agronomic applications marketed
PRACTICE
- Microbiology
- Preparation of culture media
- Cultivation of microorganisms
- Microscopic observation of microorganisms
- bacterial transformation
- Industrial biotechnology
- Management fermentors
- Plant biotechnology
- Preparation of culture media
- Set up of in vitro culture
- Micropropagation of potato
- Organogenesis in tobacco
- Expression of a reporter gene in transgenic plants
Descriptors
General principles of biology of microorganisms, bacterial genetics and metabolism, Major groups of microorganisms, microbial biotechnology, in vitro Plant tissue culture, micropropagation
General proficiencies
- CB1: Students are able to demonstrate they have acquired knowledge and understanding in a field of study based on the basic foundations gained within their general secondary education together with the support of advanced textbooks and aspects of the latest advances in the field.
- CB3: Students have the ability to collect and interpret relevant data (generally within their field of study) in order to make judgments which include reflection on relevant issues of social, scientific or ethical nature.
Specific proficiencies
- CE12: Ability to recognize the applications of biotechnology in agriculture and livestock engineering
Learning outcomes
R1 - To understand the functions of microorganisms in relation to plants and environment for their use in crop production and in agro-industrial processes
R2 - To know, understand and apply the molecular underpinnings and biotechnological techniques in the transformation of species of agronomic interest.
RESULTADOS APRENDIZAJE ENAEE:
ENAEE-11: La capacidad de diseñar y realizar experimentos, interpretar los resultados y sacar conclusiones.
Methodology
| Methodology - Activity | Classroom hours | Non-classroom hours |
| A-1 Lectures | 30 | |
| A-2 Practice | 30 | 10 |
| A-3 Debates, knowledge pooling, group mentoring | 7 | |
| A-4 Homework | 20 | |
| A-5 Reading Material | 20 | |
| A-6 Personal study | 30 | |
| A-7 Exams, assessment tests | 3 | |
| A-8 Indivual mentoring | ||
| ¿ | ||
| Total | 63 | 87 |
Evaluation
| Learning outcome | Evaluation system | Weight(%) | Recoverability |
| R1, R2 | Written test of the theoretical acquired concepts | 70% minimum grade for weigh in final score = 5/10 in this section |
Recoverable, written test |
| R1, R2 | Written work submitted by the students | 25% minimum grade for weigh in final score = 5/10 in this section |
Recoverable, written test |
| R1, R2 | Record of attendance | 5% | No |
To pass the course, it is necessary, but not sufficient, to pass the course exam (score equal to or greater than 5.0) and to attend at least to 80% of the practical classes. Should not justify the practical classes attendance requirements, it will be necessary, but not sufficient, to approve an special practical course exam in addition to the general course exam.
The students failing the practical exam, will have the mark of that practical exam as final course mark.
Agenda
THEORY
- General Microbiology
- Structure microorganisms
- Bacteria
- Mushrooms
- Virus
- Metabolism (primary and secondary metabolism)
- Primary Metabolism
- Secondary metabolism
- Growth kinetics of growth and culture media
- Elements molecular biology
- Structure of genetic material and
- Regulation of gene expression in prokaryotes and eukaryotes
- Manipulation of genetic material
- Transformation
- Conjugation
- Microbial groups of interest
- Proteobacteria
- Firmicutes
- Actinobacteria
- Mushrooms
- Relationships between different organisms
- Rhizobium
- Agrobacterium
- Ruminal ecosystem
- Industrial microbiology
- Products of bacterial origin
- Products of fungal origin
- Fermenters and bioreactors
- Waste treatment
- Solid wastes
- Liquid wastes
- Bioremediation
- Plant biotechnology
- General micropropagation techniques
- Facilities
- Culture media
- Multiplication by nodal segments or axillary branching
- Organogenesis
- Somatic embryogenesis
- Commercial Applications
- Production of virus-free plants
- Conservation of germplasm in vitro
- Medium-term conservation
- Stable long-term cryopreservation
- Other applications of plant tissue culture
- Protoplast culture and somatic hybridization
- Haploid Obtention
- Production of secondary metabolic compounds
- Transgenic Plants
- Concepts and methods of obtention
- Agronomic applications marketed
PRACTICE
- Microbiology
- Preparation of culture media
- Cultivation of microorganisms
- Microscopic observation of microorganisms
- bacterial transformation
- Industrial biotechnology
- Management fermentors
- Plant biotechnology
- Preparation of culture media
- Set up of in vitro culture
- Micropropagation of potato
- Organogenesis in tobacco
- Expression of a reporter gene in transgenic plants
Bibliography
Access the bibliography that your professor has requested from the Library.
MICROBIOLOGÍA y BIOTECNOLOGÍA INDUSTRIAL
Madigan MT, Martinko JM Dunlap, PV y Clark, DP. 2009. Brock: Biología de los microorganismos. Ed. Pearson, Madrid.
Prescott LM, Harley JP y Klein DA. 2002. Microbiología. McGraw-Hill Interamericana de España. Madrid.
BIOTECNOLOGÍA VEGETAL
Hurtado DV y Merino ME (eds). 1987. Cultivo de tejidos vegetales. Editorial Trillas, México.
Lindsey K y Jones MGK. 1992. Biotecnología vegetal agrícola. Editorial Acribia, Zaragoza.
Serrano M y Piñol MT. 1991. Biotecnología Vegetal. Editorial Síntesis, Madrid.
Vidalie H (ed). 1986. Cultivo in vitro. Editorial Científica, México.
Trigiano RN y Gray DJ (eds). 2000. Plant tissue culture concepts and laboratory exercises. CRC Press, Boca Ratón.