Public University of Navarre



Castellano | Academic year: 2014/2015
Bachelor's degree in Agricultural, Food and Rural Environment Engineering at the Universidad Pública de Navarra
Course code: 501304 Subject title: MICROBIOLOGY AND PLANT BIOTECHNOLOGY
Credits: 6 Type of subject: Mandatory Year: 2 Period: 1º S
Department: Agrarian Production
Lecturers:
PISABARRO DE LUCAS, ANTONIO GERARDO   [Mentoring ] VERAMENDI CHAROLA, JON MIRENA   [Mentoring ]
LARRAYA RETA, LUIS MARIA   [Mentoring ] OGUIZA TOME, JOSE ANTONIO   [Mentoring ]

Partes de este texto:

 

Contents

THEORY

  1.  General Microbiology
    1. Structure microorganisms
      1. Bacteria
      2. Mushrooms
      3. Virus
    2. Metabolism (primary and secondary metabolism)
      1. Primary Metabolism
      2. Secondary metabolism
    3. Growth kinetics of growth and culture media
    4. Elements molecular biology
      1. Structure of genetic material and
      2. Regulation of gene expression in prokaryotes and eukaryotes
    5. Manipulation of genetic material
      1. Transformation
      2. Conjugation
    6. Microbial groups of interest
      1. Proteobacteria
      2. Firmicutes
      3. Actinobacteria
      4. Mushrooms
    7. Relationships between different organisms
      1. Rhizobium
      2. Agrobacterium
      3. Ruminal ecosystem
  2. Industrial microbiology
    1. Products of bacterial origin
    2. Products of fungal origin
    3. Fermenters and bioreactors
    4. Waste treatment
      1. Solid wastes
      2. Liquid wastes
    5. Bioremediation
  3. Plant biotechnology
    1. General micropropagation techniques
      1. Facilities
      2. Culture media
      3. Multiplication by nodal segments or axillary branching
      4. Organogenesis
      5. Somatic embryogenesis
      6. Commercial Applications
    2. Production of virus-free plants
    3. Conservation of germplasm in vitro
      1. Medium-term conservation
      2. Stable long-term cryopreservation
    4. Other applications of plant tissue culture
      1. Protoplast culture and somatic hybridization
      2. Haploid Obtention
      3. Production of secondary metabolic compounds
    5. Transgenic Plants
      1. Concepts and methods of obtention
      2. Agronomic applications marketed

 

PRACTICE

  1. Microbiology
    1. Preparation of culture media
    2. Cultivation of microorganisms
    3. Microscopic observation of microorganisms
    4. bacterial transformation
  2. Industrial biotechnology
    1. Management fermentors
  3. Plant biotechnology
    1. Preparation of culture media
    2. Set up of in vitro culture
    3. Micropropagation of potato
    4. Organogenesis in tobacco
    5. Expression of a reporter gene in transgenic plants

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Descriptors

General principles of biology of microorganisms, bacterial genetics and metabolism, Major groups of microorganisms, microbial biotechnology, in vitro Plant tissue culture, micropropagation

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General proficiencies

CT1: Ability to develop activities in the field of Agricultural and Rural Engineering assuming a social, ethical and sustainable commitment

 

CT2: Ability for effective oral and written communication in Spanish

 

CT3: Ability for effective oral and written communication in English

 

CT7: Ability to solve problems with creativity, initiative and critical thinking methodology

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Specific proficiencies

CE12: Ability to recognize the applications of biotechnology in agriculture and livestock engineering

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Methodology

Methodology - Activity

Classroom hours

Non-classroom hours

A-1 Lectures

30

 

A-2 Practice

30

10

A-3 Debates, knowledge pooling, group mentoring

 

7

A-4 Homework

 

20

A-5 Reading Material

 

20

A-6 Personal study

 

30

A-7 Exams, assessment tests

3

 

A-8 Indivual mentoring

 

 

…

 

 

Total

63

87

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Evaluation

Topic

Criteria

Assessment tool

Weight

Theoretical knowledge

Mastery of theoretical knowledge

Course exam

50 %

Practical knowledge

Mastery of practical skills associated with the subject

Practical case study and solution

25 %

 

 

Comprehension and synthesis of literature

Collection, compilation and presentation of information related to the subject

Preparation and presentation of a literature-based work

15 %

 

Class participation

Attendance and participation in classroom activities

Student attendance and participation files

5 %

 

 

To pass the course, it is necessary, but not sufficient, to pass the course exam (score equal to or greater than 5.0) and to attend at least to 80% of the practical classes. Should not justify the practical classes attendance requirements, it will be necessary, but not sufficient, to approve an special practical course exam in addition to the general course exam.

 

The students failing the practical exam, will have the mark of that practical exam as final course mark.

 

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Bibliography

Access the bibliography that your professor has requested from the Library.


MICROBIOLOGÍA y BIOTECNOLOGÍA INDUSTRIAL

 Madigan MT, Martinko JM Dunlap, PV y Clark, DP. 2009. Brock: Biología de los microorganismos. Ed. Pearson, Madrid.

 Prescott LM, Harley JP y Klein DA. 2002. Microbiología. McGraw-Hill Interamericana de España. Madrid.

BIOTECNOLOGÍA VEGETAL

 Hurtado DV y Merino ME (eds). 1987. Cultivo de tejidos vegetales. Editorial Trillas, México.

 Lindsey K y Jones MGK. 1992. Biotecnología vegetal agrícola. Editorial Acribia, Zaragoza.

 Serrano M y Piñol MT. 1991. Biotecnología Vegetal. Editorial Síntesis, Madrid.

 Vidalie H (ed). 1986. Cultivo in vitro. Editorial Científica, México.

 Trigiano RN y Gray DJ (eds). 2000. Plant tissue culture concepts and laboratory exercises. CRC Press, Boca Ratón.

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Languages

English / Spanish

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Location

Lecture Building (Aulario)

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